Biophysical Chemistry and Nuclear Magnetic Resonance

David Weliky

Professor

41 CEM

517-353-1177


Research webpage


Primary Research Area

Physical (Ph)

Other Area(s) of Interest

Analytical (An)

Biological (Bi)

Chemical Physics (CP)

Inorganic (In)

Material (Ma)

Research

(Research Description PDF)

Structures of the influenza virus HA2 fusion peptide in membrane deduced from our NMR data.
Structures of the influenza virus HA2 fusion peptide in membrane deduced from our NMR data.

We are a biophysical chemistry group that is focused on understanding the mechanism of entry by viruses enveloped by a membrane. Many important human pathogens are enveloped viruses, including Human Immunodeficiency (HIV), Influenza, Measles, Rabies, West Nile, Zika, Ebola, SARS, and MERS. Each virus has evolved a protein in its membrane that catalyzes the joining (“fusion”) of the virus membrane with the membrane of the target cell. We are studying the glycoprotein 41 kDa (gp41) fusion protein of HIV and hemagglutinin subunit 2 (HA2) fusion protein of influenza. Our work and contributions include the protein structures and locations in membrane. We also study the changes in membrane structure associated with the protein. A significant fraction of our effort is in development and application of “solid-state”, i.e. anisotropic nuclear magnetic resonance (NMR) to these proteins. We also apply a variety of other biophysical methods including circular dichroism spectroscopy, fluorescence spectroscopy, hydrogen-deuterium exchange mass spectrometry, X-ray crystallography, and electron microscopy. We also have a significant effort in protein synthesis and chromatographic purification that includes molecular biology and protein expression in bacteria, solid-phase peptide synthesis, and native chemical ligation. A side-project in the laboratory is NMR analysis of expressed proteins in bacterial inclusion bodies, which are commonly-formed solid protein aggregates. We want to understand why these aggregates form, and the degree of folding of individual proteins within the aggregates. Our NMR methodology focuses on quantitative determination of distributions of populations of protein structures and membrane locations, with a particular emphasis on the rotationalecho double-resonance (REDOR) approach which is robust and amenable to quantitative analysis.

Models for influenza HA2-mediated membrane apposition based on our experimental data.
Models for influenza HA2-mediated membrane apposition based on our experimental data.

 

Selected Publications

Hydrogen-Deuterium Exchange Supports Independent Membrane-Interfacial Fusion Peptide and Transmembrane Domains in Subunit 2 of Influenza Virus Hemagglutinin Protein, a Structured and Aqueous-Protected Connection between the Fusion Peptide and Soluble Ectodomain, and the Importance of Membrane Apposition by the Trimerof- Hairpins Structure, A. Ranaweera, P. U. Ratnayake, E. A. P. Ekanayaka, R. Declercq, and D. P. Weliky, Biochemistry 2019, 58, 2432-2466.

The Stabilities of the Soluble Ectodomain and Fusion Peptide Hairpins of the Influenza Virus Hemagglutinin Subunit II Protein Are Positively Correlated with Membrane Fusion, A. Ranaweera, P. U. Ratnayake, and D. P. Weliky, Biochemistry 2018, 57, 5480-5493. Efficient Fusion at Neutral pH by Human Immunodeficiency Virus gp41 Trimers Containing the Fusion Peptide and Transmembrane Domains, S. Liang, P. U. Ratnayake, C. Keinath, L. Jia, R. Wolfe, A. Ranaweera, and D. P. Weliky, Biochemistry 2018, 57, 1219-1235.

Closed and Semiclosed Interhelical Structures in Membrane vs Closed and Open Structures in Detergent for the Influenza Virus Hemagglutinin Fusion Peptide and Correlation of Hydrophobic Surface Area with Fusion Catalysis, U. Ghosh, L. Xie, L. Jia, S. Liang, and D. P. Weliky, J. Am. Chem. Soc. 2015, 137, 7548-7551.

REDOR Solid-State NMR as a Probe of the Membrane Locations of Membrane- Associated Peptides and Proteins, L. Jia, S. Liang, K. Sackett, L. Xie, U. Ghosh, and D. P. Weliky, J. Mag. Res. 2015, 253, 154-165.

CV

B.A., 1985, Swarthmore College

Ph.D., 1995, Univ. of Chicago

Postdoctoral Fellow, 1995-97, National Institutes of Health.

Awards/Honors

1998 New Faculty Award Camille and Henry Dreyfus Foundation
1996 NIH Fellows Award for Research Excellence National Institutes of Health
1995 - 1997 Postdoctoral Research Fellow National Institutes of Health
1995 Ph.D. The University of Chicago
1992 Research Fellow AT&T Bell Laboratories
1991 - 1995 AT&T Ph.D. Scholar AT&T
1988 - 1991 NSF Predoctoral Fellow National Science Foundation
1987 - 1991 McCormick Fellow
1985 Bachelor of Arts with High Honors in Chemistry and Physics Swarthmore College
1985 Member Sigma Xi Honor Society (Swarthmore College)
1985 Phi Beta Kappa Phi Beta Kappa (Swarthmore College)
1981 - 1982 N. Harvey Collisson Scholar Swarthmore College
1981 - 1985 National Merit Scholar