CURRICULUM VITAE

 

John L. McCracken

 

Personal Information

 

            Place of birth:              Decatur, Illinois

            Marital Status:             Married, 4 children

 

Education

 

            B. S. in Chemistry, University of Illinois; Urbana, Illinois                                     1978

            Ph.D. in Chemistry, University of California; Berkeley, California                       1983

 

Professional Experience

 

            Chair, Department of Chemistry                                                                2002-present

            Professor of Chemistry, Michigan State University                                   2000-present

            Associate Chair for the Graduate Program, MSU                                         2000-2002

            Associate Professor of Chemistry, Michigan State University                      1995-2000

            Assistant Professor of Chemistry, Michigan State University                       1990-1995

            Lecturer in Biophysics, Albert Einstein College of Medicine                       1988-1990

            Associate Director, NIH Pulsed EPR Research Resource                                             

                 Albert Einstein College of Medicine, New York                                     1985-1990

            Postdoctoral Fellow, Albert Einstein College of Medicine, New York        1983-1985

            Graduate Student Research Assistant, University of California                   1978-1983

 

Educational Awards and Honors

 

            Edmund James Scholar, University of Illinois                                               1974-1978

            Bachelor of Science with Highest Distinction in Chemistry,

                 University of Illinois                                                                                          1978

            Chancellor's Fellow in Chemistry, University of California                                   1981

            Michigan State University, College of Natural Sciences

                       Teacher-Scholar Award                                                                                1995

            Michigan State University Teacher-Scholar Award                                                1997

 

Professional Society Memberships

            American Chemical Society

            American Physical Society

            International EPR Society

            International Society of Magnetic Resonance

Professional Service

            NIH National Pulsed EPR Research Resource Governing Board                 1985-1999

            Member, NIH Special Study Sections                                     1990-91, 1995-6,2000-7

            Member, NIH Metallobiochemistry Study Section                                       1996-2000       

            Officer, MSU Local Section of the ACS                                                            1992-5

Publications

 

1.         Friesner, R.; McCracken, J.; and Sauer, K.  Transient solutions to the Bloch equations for inhomogenously broadened lines.  J. Magn. Reson. 43, 343-356 (1981).

 

2.         McCracken, J.; Frank, H.; and Sauer, K.  Radical pair interactions in spinach chloroplasts.  Biochim. Biophys. Acta 679, 156-168 (1982).

 

3.         McCracken, J. and Sauer, K.  Orientation dependence of radical pair interactions in spinach chloroplasts.  Biochim. Biophys. Acta 724, 83-93 (1983).

 

4.         McCracken, J. and Sauer, K.  Electron paramagnetic resonance studies of the primary electron acceptors of photosystem I.  In:  Proceedings of the 6th International Congress on Photosynthesis, Brussels, Belgium (1983).

 

5.         Malikayil, J. A.; Sweeney, W. V.; McCracken, J.; and Peisach, J.  The lack of a solvent accessible hydroxide or water ligand to iron at the 3Fe center of Azotobacter Vinelandii ferredoxin I.  Biochem. Biophys. Res. Commun. 133, 1119-1124 (1985).

 

6.         McCracken, J.; Peisach, J.; and Dooley, D. M.  Cu(II) coordination chemistry of amine oxidases:  Pulsed EPR studies of histidine imidazole, water, and exogenous ligand coordination.  J. Am. Chem. Soc. 109, 4064-4072 (1987).

 

7.         Dooley, D. M.; McGuirl, M. A.; Peisach, J.; and McCracken, J.  The generation of an organic free radical in substrate-reduced pig kidney diamine oxidase-cyanide.  FEBS Lett. 214, 274-278 (1987).

 

8.         Magliozzo, R. S.; McCracken, J.; and Peisach, J.  Electron-nuclear coupling in nitrosyl heme proteins and in nitrosyl ferrous and oxy cobaltous tetraphenylporphyrin complexes.  Biochemistry 26, 7923-7931 (1987).

 

9.         Mondovi, B.; Morpurgo, L.; Agostinelli, E.; Befani, O.; McCracken, J.; and Peisach, J.  A comparison of the local environment of Cu(II) in holo and half Cu-depleted bovine serum amine oxidase.  Eur. J. Biochem. 168, 503-507 (1987).

 

10.       McCracken, J.; Pember, S.; Benkovic, S. J.; Villafranca, J. J.; Miller, R.; and Peisach, J.  Electron spin echo studies of the Cu(II) site in phenylalanine hydroxylase from Chromobacterium violaceum.  J. Am Chem. Soc. 110, 1069-1074 (1988).

 

11.       Doi, K.; McCracken, J.; Peisach, J.; and Aisen, P.  The binding of molybdate to uteroferrin:  hyperfine interactions of the binuclear center with 95Mo, 1H, and 2H.  J. Biol. Chem. 263, 5757-5763 (1988).

 

12.       McCracken, J.; Desai, P. R.; Papadopoulos, N. J.; Villafranca, J. J.; and Peisach, J.  Electron spin-echo studies of the copper(II) binding sites in dopamine B-hydroxylase.  Biochemistry 27, 4133-4137 (1988).

 

13.       Traylor, T. G.; Hill, K. W.; Tian, Z.; Rheingold, A. L.; McCracken, J.; and Peisach, J.  Ene diimidazoles:  Metal ligands for biomimetic chemistry.  J. Am. Chem. Soc. 110, 5571-5573 (1988).

 

14.       Cammack, R.; Chapman, A.; McCracken, J.; Cornelius, J. B.; Peisach, J.; and Weiner, J. H.  Electron spin-echo spectroscopic studies of E. coli fumarate reductase.  Biochim. Biophys. Acta 956, 307-312 (1988).

 

15.       Serpersu, E. H.; McCracken, J.; Peisach, J.; and Mildvan, A. S.  Electron spin-echo envelope modulation and nuclear relaxation studies of staphylococcal nuclease and its metal-coordinating mutants.  Biochemistry 27, 8034-8044 (1988).

 

16.       Chapman, A.; Cammack, R.; Hatchikian, C. E.; McCracken, J.; and Peisach, J.  A pulsed EPR study of redox-dependent hyperfine interactions for the nickel centre of Desulfovibrio gigas hydrogenase.  FEBS Lett. 242, 134-138 (1988).

 

17.       Chasteen, N. D.; Snetsinger, P. A.; Swope, S. K.; van Willigen, H.; Cornelius, J. B.; and McCracken, J.  The structure of mixed-ligand cyano complexes of transferrin.  In:  UCLA Symposia on Molecular and Cellular Biology, Vol. 98 (Winge, D. and Hamer, D., eds.) Alan R. Liss, Inc., pp. 161-168 (1988).

 

18.       McCracken, J.; Cornelius, J. B.; and Peisach, J.  Quantitative aspects of nitrogen-14 electron spin-echo envelope modulation in Cu(II)-proteins.  In:  Pulsed EPR:  A New Field of Applications, (C. P. Keijzers, E. J. Riejerse, and J. Schmidt, Eds.) North Holland Publ., Amsterdam, pp. 155-161 (1989).

 

19.       Tipton, P. A.; McCracken, J.; Cornelius, J. B.; and Peisach, J.  Electron spin-echo envelope modulation studies of pyruvate kinase active site complexes.  Biochemistry 28, 5720-5728 (1989).

 

20.       Cammack, R.; Kovacs, K.; McCracken, J.; and Peisach, J.  Spectroscopic characterization of the nickel and iron-sulfur clusters of hydrogenase from the purple photosynthetic bacterium Thiocapsa roseopersicina.  Eur. J. Biochem. 182, 363-366 (1989).

 

21.       Cunningham, R. P.; Asahara, H.; Bank, J. F.; Scholes, C. P.; Salerno, J. C.; Surerus, K.; Munck, E.; McCracken, J.; Peisach, J.; and Emptage, M. H.  Endonuclease III is an iron-sulfur protein.  Biochemistry 28, 4450-4455 (1989).

 

22.       Morgan, T. V.; McCracken, J.; Orme-Johnson, W. H.; Mims, W. B.; Mortenson, L. E.; and Peisach, J.  Pulsed electron paramagnetic resonance studies of the interaction of Mg-ATP and D2O with the iron protein of nitrogenase.  Biochemistry 29, 3077-3081 (1990).

 

23.       Cornelius, J. B.; McCracken, J.; Clarkson, P. B.; Belford, R. L.; and Peisach, J.  ESEEM Angle Selection Studies of Axial Pyridine Coordination to Cu(II) Benzoylacetonate.  J. Phys. Chem. 94, 6977-6982 (1990).

 

24.       Jiang, F.; McCracken, J.; and Peisach, J.  Nuclear quadrupole interactions in Cu(II) diethylenetriamine-substituted imidazole complexes and in Cu(II) proteins.  J. Am. Chem. Soc. 112, 9035-9044 (1990).

 

25.       McCracken, J.; Peisach, J.; Bahattacharyya, L.; and Brewer, F.  Electron Spin Echo Envelope Modulation Studies of Lectins:  Evidence for a Conserved Mn(II) Binding Site.  Biochemistry 30, 4486-4491(1991).

 

26.       Cammack, R.; Chapman, A.; Williams, R.; McCracken, J.; and Peisach, J.  Electron Spin-Echo Spectroscopy of the Iron-Sulfur Clusters of Xanthine Oxidase from Milk.  J. Chem. Soc., Faraday Trans., 87, 3203-3206 (1991).

 

27.       McCracken, J.; Shin, D.-H.; Dye, J. L.  Characterization of Electron Trapping Sites in Cesium Hexamethyl Hexacyclen Sodide.  Applied Magnetic Resonance 3, 305-316 (1992).

 

28.       McCracken, J.; Peisach, J.; Cote, C. E.; McGuirl, M. A.; Dooley, D. M.  Pulsed EPR Studies of the Semiquinone State of Copper-Containing Amine Oxidases.  J. Am. Chem. Soc. 114, 3715-3720 (1992).

 

29.       Lu, J.; Bender, C. J.; McCracken, J.; Peisach, J.; Severns, J. C.; McMillan, D. R.  Pulsed EPR Studies of the Type 2 Copper Binding Site in the Mercury Derivative of Laccase.  Biochemistry 31, 6265-6272 (1992).

 

30.       Gurbiel, R. J.; Peoples, R.; Doan, P. E.; Cline, J. F.; McCracken, J.; Peisach, J.; Hoffman, B. M.; Valentine, J. S.  ENDOR and ESEEM Investigation of Ag(I)2Cu(II)2 Bovine Superoxide Dismutase.  Inorganic Chemistry  32, 1813-1819 (1993).

 

31.       Warncke, K.; Brooks, H. B.; Babcock, G. T.; Davidson, V. L.; McCracken, J.  The Nitrogen Atom of Substrate Methylamine is Incorporated into the Tryptophan Tryptophyl-Semiquinone Catalytic Intermediate in Methylamine Dehydrogenase.  J. Am. Chem. Soc. 115, 6464-5 (1993).

 

32.       Salowe, S.; Bollinger, J. M.; Ator, M.; Stubbe, J.; McCracken, J.; Peisach, J.; Samano, M. C.; Robins, M. J.; An Alternative Model for Mechanism-Based Inhibition of E. Coli Ribonucleotide Reductase by 2˘ Azido-2˘ deoxyuridine 5˘-diphosphate,  Biochemistry,  32, 12749-12760 (1993).

 

33.       Jang, S. H.; Lee, H.-I.; McCracken, J.; Jackson, J. E.  Pulsed EPR Studies of Ion Binding in a Double-Faced Paramagnetic Ionophore:  Tris (2.6-di(methoxyethoxy)phenyl) methyl Radical.  J. Am. Chem. Soc., 115, 12623-4 (1993).

 

34.       McCracken, J. and Freidenberg, S., Electron Spin Echo Envelope Modulation Studies of Water Bound to Tetracyanonickelate (III), J. Phys. Chem., 98, 467-473 (1994).

 

35.       Warncke, K.; Babcock, G. T.; Dooley, D. M.; McGuirl, M.A.  and McCracken, J., Structure of the Topaquinone Catalytic Intermediate of Methylamine Oxidase as Revealed by Magnetic Interactions with Exchangeable 2H and 1H Nuclei, J. Am. Chem. Soc., 116, 4028-4037 (1994).

 

36.       Warncke, K. and McCracken, J., 2H Electron Spin Echo Envelope Modulation Spectroscopy of Strong a-Hydrogen Hyperfine Coupling in Randomly-oriented Paramagnetic Systems , J. Chem. Phys.,  101, 1832-1841, 1994.

           

37.       Warncke, K.; McCracken, J. and Babcock, G.T. Structure of the YD Tyrosine Radical in Photosystem II as Revealed by 2H Electron Spin Echo Envelope Modulation Spectroscopic Analysis of Hydrogen Hyperfine Interactions, J. Am. Chem Soc., 116, 7332-7340, 1994.

 

38.       Lee, H-I. and McCracken, J. Four-Pulse Electron Spin Echo Envelope Modulation Studies of Axial Water Ligation to Bis-Aquo Tetracyanonickelate(III), J. Phys. Chem., 98,12861-12870, 1994.

 

39.       Magliozzo, R.S.; Bubacco, L.; McCracken, J.; Jiang, F.; Beltramini, M.; Salvatto, B. and Peisach, J. Cu(II) Coordination in Arthropod and Mollusc Green Half-Met Hemocyanins Analyzed by Electron Spin Echo Envelope Modulation Spectroscopy, Biochemistry,  34, 1513-1523, 1995.

 

40.       Kumar, M.; Lu, W-P; Smith, A.; Ragsdale, S.W.; and McCracken, J.  Azide Binding to the Site of CO Oxidation by Carbon Monoxide Dehydrogenase from Clostridium thermoaceticum, J. Am. Chem. Soc., 117, 2939-2940, 1995.

 

41.       Espe, M.P.; Hosler, J.P.;  Ferguson-Miller, S.; Babcock, G.T.; and McCracken, J.  A CW- and Pulsed EPR Characterization of the Mn(II) Binding Site in Rhodobacter Sphaeroides Cytochrome c Oxidase, Biochemistry  34, 7593-7602, 1995.

 

42.       Hoganson, C.W.; Lydakis-Simantiris, N.; Tang, X-S.; Tommos, C.; Warncke, K.; Babcock, G.T.; Diner, B.A.; McCracken, J. and Styring, S. A Hydrogen Abstraction Model for the Function of YZ in Photsynthetic Oxygen Evolution, Photosynthesis Research  46, 177-184, 1995.

 

43.       Warncke, K.; Brooks, H.B.; Lee, H-I.; McCracken, J.; Davidson, V.L. and Babcock, G.T.   Structure of the Dithionite-Generated Tryptophan Typtophylquinone Cofactor Radical in Methylamine Dehydrogenase Revealed by ENDOR and ESEEM Spectroscopies, J. Am. Chem. Soc., 117, 10063-10075, 1995.

 

44.       Warncke, K. and McCracken, J. Analysis of Static Distributions in Hydrogen Hyperfine Interactions in Randomly Oriented Radicals in the Solid State by Using Deuterium Electron Spin Echo Envelope Modulation Spectroscopy: Conformational Dispersion of b-2H Coupling in the Model Tyrosyl Radical, J. Chem. Phys., 103, 6829-6840, 1995.

 

45.       Tommos, C.; Tang, X-S.; Warncke, K.; Hoganson, C.; Styring, S.; McCracken, J.; Diner, B.A. and Babcock, G.T.  Spin Density Distribution, Conformation, and Hydrogen Bonding of the Redox-Active Tyrosine YZ in Photosystem II from Multiple Electron Magnetic Resonance Spectroscopies: Implications for Photosynthetic Oxygen Evolution, J. Am. Chem. Soc., 117, 10325-10335, 1995.

 

46.       Theodorakis, J.L.; Garber, E.A.E.; McCracken, J.; Peisach, J.; Schejter, A.; Margoliash, E. A Chemical Modification of Cytochrome-c Lysines Leading to Changes in Heme Iron Ligation, Biophys. Biochim. Acta 1252, 103-113, 1995.

 

47.       Warncke, K.; Babcock, G.T. and McCracken, J. Static Conformational Distributions in the Solid State: Analysis and Application to Angular Dispersion in Side Chain Orientations in Model Tyrosine in Aqueous Glass," J. Phys. Chem. 100, 4654-4661, 1996.

 

48.       Mac, M.; Tang, X-S.; Diner, B.A.; McCracken, J.; Babcock, G.T. Identification of Histidine as an Axial Ligand to P700+, Biochemistry,  35, 13288-13293, 1996.

 

49.       Babcock, G.T.; Espe, M.; Hoganson, C.; Lydakis-Simantiris, N.; McCracken, J.; Shi, W.; Styring, S.; Tommos, C. and Warncke, K.  Tyrosyl Radicals in Enzyme Catalysis: Some Properties and a Focus on Photosynthetic Water Oxidation, Acta  Chem. Scand.,   51, 533-540, 1997.

 

50.       Whiting, A.K.; Que, L.; Wallace, R.E.; Hausinger, R.P.; Frederick, M.A.; and McCracken, J. Metal Coordination Environment of a Cu(II)-Substituted a-Keto Acid-Dependent Dioxygenase That Degrades the Herbicide 2,4-D, J. Am. Chem. Soc.,  119, 3413-3414, 1997.

 

51.       Colpas, G.J.; Brayman, T.G.; McCracken, J.; Pressler, M.A.; Babcock, G.T.; Ming, L-J; Colangelo, C.M.; Scott, R.A. and Hausinger, R.P. Spectroscopic Characterization of Metal Binding by Klebsiella aerogenes UreE Urease Accessory Protein, J. Biol. Inorg. Chem., 3, 150-160, 1998.

 

52.       Kosman, D.J.; Hassett, R.; Yuan, D.S. and McCracken, J. Spectroscopic Characterization of the Cu(II) Sites in the Multinuclear Copper Oxidase from Yeast, the FET-3 Protein J. Am. Chem. Soc., 120, 4037-8, 1998.

 

53.       Tommos, C.; McCracken, J.; Styring, S; Babcock, G.T. Stepwise Disintegration of the Photosynthetic Oxygen Evolving Complex J. Am Chem Soc., 120, 10441-10452  , 1998.

 

54.       Tommos, C; Hoganson, C.W.; Di Valentin, M.; Lydakis-Simantiris, N.; Dorlet, P.; Westphal, K.; Chu, H-A; McCracken, J. and Babcock, G.T. Manganese and Tyrosyl Radical Function in Photosynthetic Oxygen Evolution Current Opinion in Chemical Biology, 2, 244-252, 1998.

 

55.       Dorlet, P.; DiValentin, M.; Babcock, G.T.; McCracken, J. Interaction of Yz° with its Environment in Acetate Treated Photosystem II Membranes and reaction Center Cores J. Phys. Chem., 102, 8239-8247, 1998.

 

56.               Mac, M.; Bowlby, N.R.; Babcock, G.T.; McCracken, J. Monomeric Spin Density Distribution in the Primary Donor of Photosystem I as Determined by Electron Magnetic Resonance: Function and Thermodynamic Implications J. Am. Chem. Soc. 120, 13215 - 13223, 1998.

 

57.              Florens, L.; Hoganson, C.; McCracken, J.; Fetter, J.; Mills, D.A.; Babcock, G.T.; Ferguson-Miller, S. The Role of Magnesium and its Associated Water Channel in Activity and Regulation of Cytochrome c Oxidase The Phototropic Procaryotes , ed. G. Peschek, Kluwer Academic/Plenum Press, New York, 329-339, 1999.

 

58.              Bouchev, V.F.; Furdui, C.M.; Menon, S.; Muthukumaran, R.B.; Ragsdale, S.W. and McCracken, J. ENDOR Studies of Pyruvate:Ferredoxin Oxidoreductase Reaction Intermediates, J. Am. Chem. Soc., 121, 3724-3729, 1999.

 

59.              Hegg, E.L.; Whiting, A.K.; Saari, R.E.; McCracken, J.; Hausinger, R.P. and Que, L. The Herbicide-Degrading a‑Keto Acid-Dependent Enzyme TfdA: Metal Coordination Environment and Mechanistic Insights.  Biochemistry, 38, 16714-16726, 1999.

 

60.              Singh, V.; Zhu, Z.; Davidson, V.L. and McCracken, J.  Characterization of the Tryptophan Tryptophyl-Semiquinone Catalytic Intermediate of Methylamine Dehydrogenase by Electron Spin Echo Envelope Modulation Spectroscopy. J. Am. Chem. Soc., 122, 931-938, 2000.

 

61.              Hogan, D.A.; Smith, S.R.; Saari, E.A.; McCracken, J. and Hausinger, R.P. Site-Directed Mutagenesis of 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase, J. Biol. Chem., 275, 12400-12409, 2000.

 

62.              Smith, T.; McCracken, J.; Shin, Y-K. and DeWitt, D.L. Arachidonic Acid and NSAIDs Induce conformational Changes In the Human Prostaglandin Endoperoxide H2 Synthase-2 (COX-2), J. Biol. Chem. 275, 40407-40415, 2000.

 

63.              Schmidt, B; Florens, L.; McCracken, J. and Ferguson-Miller, S. Fast Deuterium Exchange to the Buried Magnesium/Manganese Site in Cytochrome-c Oxidase, Biochemistry, 40, 7491-7497, 2001.

 

64.              Artz, J.D; Schmidt, B; McCracken, J.L. and Marletta, M.A. Effects of nitroglycerin on soluble Guanylate Cyclase: Implications for Nitrate Tolerance, J. Biol. Chem., 277, 18253-18256, 2002.

 

65.              Ryle, M.J.; Liu, A.; Muthukumaran, R.B.; Ho, R.Y.N.; McCracken, J.; Que, L. and Hausinger, R.P. "Tyrosyl Radical Formation in TauD, an Alpha-Keto Acid Dependent Non-heme Iron Dioxygenase,"  Biochemistry, 42, 1854-1862, 2003.

 

66.              Schmidt, B.; McCracken, J. and Ferguson-Miller, S."A discrete water exit pathway for the membrane protein, cytochrome c oxidase,"  Proc. Natl. Acad. Sci.,  100, 15539-15542, 2003.

 

67.              Schmidt, B.; Hillier, W., McCracken, J. and Ferguson-Miller, S. “The use of stable isotopes to investigate the energy transducing protein cytochrome c oxidase,” Biochimica Biophysica Acta,  1655, 248-255, 2004.

 

68.              Bernards, M.L.; Thelen, K.D.; Penner, D.; Muthukumaran, R.B. and McCracken, J. “Glyphosate Interaction with Manganese in Tank Mixtures and its Effect of Glyphosate Adsorption and Translocation,”Weed Science,  53, 787-794, 2005.

 

69.              MirAfzali, Z.; Leipprandt, J.R.; McCracken, J.L.; and DeWitt, D.L. “Fast, Efficient Reconstitution of  the cyclooxygenases into Proteoliposomes,” Arch. Biochem. Biophys. 443, 60-65, 2005.

 

70.              MirAfzali, Z.; Leipprandt, J.R.; McCracken, J.L.; and DeWitt, D.L. “Topography of the Prostaglandin Endoperoxide H2 Synthase-2 in Membranes,” J. Biol. Chem., 281, 28354-29364, 2006.

 

71.              Muthukumaran, R.B.; Grzyska, P.K.; Hausinger, R.P. and McCracken, J. “Probing the Fe-substrate orientation for taurine/a-ketoglutarate dioxygenase using deuterium ESEEM spectroscopy,” Biochemistry, 46, 5951-5959, 2007.

 

72.              McCracken, J.; Vassiliev, I.R.; Yang, E.C.; Range, K. and Barry, B.A. “ESEEM Studies of Peptide Nitrogen Hyperfine Coupling in Tyrosyl Radicals and Model Peptides,” J. Phys. Chem. B, 2007, in press.

 

73.              Valayutham, M.; Muthukumaran, R.B.; Sostaric, J.Z.; McCracken, J. and Zweier, J.L. “A Novel Pathway for Cancer Chemoprevention : Role of Cytochrome c,” Free Radical Biology and Medicine, 2007, in press.

 

 

Book Chapters and Reviews

 

1.                  McCracken, J. Electron Spin Echo Envelope Modulation, Handbook of Electron Spin Resonance, Volume II, C.P. Poole and H.A. Farach, eds., Springer-Verlag, New York, 1999, Chapter V.

 

2.                  McCracken, J. Electron Spin Echo Envelope Modulation, Encyclopedia of Inorganic Chemistry, volume 2, R.A. Scott and C.M. Lukehart, eds., John Wiley & Sons, Ltd, West Sussex, UK, 2007, in press.

 

 

 

Invited Talks

 

1.         "Radical Pair Interactions in Spinach Chloroplasts," Procter and Gamble Co., Cincinnati, Ohio, February 1983.

 

2.         "Time-Resolved EPR Studies of Photosynthetic Electron Transport," Medical College of Wisconsin, Milwaukee, Wisconsin, February 1983.

 

3.         "Time-Resolved EPR Studies of Photosynthetic Electron Transport," IBM Instruments, Danbury, Connecticut, February 1983.

 

4.         "Orientation Dependence of Radical Pair Interactions in Photosystem I," Bell Telephone Laboratories, Murray Hill, New Jersey, February 1983.

 

5.         "Pulsed EPR Studies of the Cu(II) Binding Site in Amine Oxidases," 29th Rocky Mountain Conference on Analytical Chiemistry, Denver, Colorado, August 1986.

 

6.         "Pulsed EPR Studies of the 2Fe-2S Cofactor in Fumarate Reductase," XIII International Conference on Magnetic Resonance in Biological Systems, Madison, Wisconsin, August 1988.

 

7.         "Pulsed EPR Studies of the Nickel Site in D. Gigas Hydrogenase," Department of Chemistry, University of New Mexico, Albuquerque, New Mexico, December 1988.

 

8.         "Pulsed EPR Studies of the Nickel Site in D. Gigas Hydrogenase," Department of Chemistry, Michigan State University, February 1989.

 

9.         "Pulsed EPR Studies of the Nickel Site in D. Gigas Hydrogenase," Department of Chemistry, University of Oklahoma, February 1989.

 

10.       "Pulsed EPR Studies of the Nickel Site in D. Gigas Hydrogenase," Department of Chemistry, Arizona State University, March 1989.

 

11.       "Electron Spin Echo Studies of Ni-Fe Hydrogenases," Fourth International Conference on Bioinorganic Chemistry, Cambridge, Massachusetts, July 1989.

 

12.       "Electron Spin Echo Studies of Ni-Fe Hydrogenases," XIV International Conference on Magnetic Resonance in Biological Systems, Warwick, U. K., September 1990.

 

13.       "Pulsed EPR Studies of Nickel Model Complexes," MSU Foundation Annual Meeting, December 1990.

 

14.       "Pulsed EPR Studies of Amine Oxidase," Wellcome Lectureship Symposium, MSU Biochemistry Department, February 1991.

 

15.       "Electron Spin Echo Envelope Modulation Studies of Ni(III) Model Complexes," 33rd Rocky Mountain Conference on Analytical Chemistry, August 1991.

 

16.       "Pulsed EPR Studies of the Cu(I)-Semiquinone State of Amine Oxidases," Department of Chemistry, Bryn Mawr College, Bryn Mawr, Pennsylvania, February 1992.

 

17.       "Pulsed EPR Studies of Nickel Model Complexes," Gordon Conference on Magnetic Resonance, Tilton School, July 1992.

 

18.       "Pulsed EPR Studies of Radical Intermediates in Biological Reactions," Department of Chemistry, Columbia University, New York, NY, 24 September 1993.

 

19.       "Pulsed EPR Studies of Radical Intermediates in Biological Reactions," Department of Chemistry, Oakland University, Rochester, MI, 29 September 1993.

 

20.       "Pulsed EPR Studies of Radical Intermediates in Biological Reactions," Department of Chemistry, Northwestern University, Evanston, IL, 1 November 1993.

 

21.       "2H Electron Spin Echo Envelope Modulation Spectroscopy of Strong a-Hydrogen Hyperfine Coupling in Randomly-oriented Paramagnetic Systems", Depts. of Chemistry and Physics, Washington University, St. Louis, MO, 25 April 1994.

 

22.       "2H Electron Spin Echo Envelope Modulation Spectroscopy of Strong a-Hydrogen Hyperfine Coupling in Randomly-oriented Paramagnetic Systems", 17th International EPR Symposium, Denver, CO, August 1-4, 1994.

 

23.       "2H Electron Spin Echo Envelope Modulation Spectroscopy of Strong a-Hydrogen Hyperfine Coupling in Randomly-oriented Paramagnetic Systems",  26th Southeastern Magnetic Resonance Conference, October, 1994.

 

24.       "Probing Radical Intermediates in Amine Oxidases", Dept. of Biochemistry, University of Nebraska, Lincoln, Nov.8, 1994.

            "Pulsed EPR Primer" , Center for Metallobiochemistry Research, University of Nebraska, Lincoln, NE, Nov.9, 1994.

 

25.       "Probing Radical Intermediates in Enzymes", Dept. of Chemistry, University of California, Davis, CA, February 16, 1995.

 

26.       "Probing Radical Intermediates in Quinoproteins", Chemical Biodynamics Division, University of California, Berkeley, CA, February 17, 1995.

 

27.       "Application of Advanced Electron Magnetic Resonance Methods to the Study of Radical Intermediates in Amine Oxidases"  Gordon Conference on Quinone and Redox-Active Amino Acid Cofactors, Ventura, CA, February, 19-24, 1995.

 

28.       "Probing Radical Intermediates in Quinoproteins", Biophysics Research Institute, Medical College of Wisconsin, March 17, 1995.

 

29.       "Biological Applications of Advanced EPR Spectroscopy", Dept. of Chemistry, University of Michigan, Oct. 3, 1996.

 

30.       "Advanced EPR Spectroscopy for Biochemistry", Dept. of Biochemistry, Michigan State University, January 10, 1997.

 

31.       "Structure of Radical Intermediates in Electron Transfer Proteins and Enzymes", Gordon Conference on Magnetic Resonance in Biological Systems, Ventura, CA, January 27-31, 1997.

 

32.       "Structure of Radical Intermediates in Electron Transfer Proteins and Enzymes",  Dept. of Physics, Emory University, March 7, 1997.

 

33.       “Biological Applications of Pulsed Electron Paramagnetic Resonance Spectroscopy”, Dept. of Chemistry, SUNY-Geneseo, September 22, 1997.

 

34.       "Structure of Radical Intermediates in Electron Transfer Proteins and Enzymes", Dept. of Chemistry, Rochester Institute of Technology, September 24, 1997.

 

35.       “Recent Advances in ESEEM Spectroscopy”, Illinois EPR Center, University of Illinois, Urbana, IL, November 6, 1997.

 

36.       "Structure of Radical Intermediates in Electron Transfer Proteins and Enzymes", Dept. of Chemistry, Kalamazoo College, November 19, 1997.

 

37.       “Orientational Distributions in the Z-Tyrosine, De-cloaking Signal II,” Sauer-Klein Symposium, University of California, Berkeley, CA, January, 1998.

 

38.              “Probing Radical Intermediates in Enzymes,” Dept. of Chemistry,  Indiana State University, March 3, 1998.

 

39.              “Simplifying EPR Spectra Using an Electron Spin Echo Magnetic Field Jump Method,” Illinois State University, Sept 11, 1998.

 

40.              “Probing Radical Intermediates in Enzymes,” Dept. of Chemistry, Boston University, March 3, 1999.

 

41.              “Characterization of the metal binding site of an a-ketoglutarate dependent iron dioxygenase,” 41st  Rocky Mountain Conference on Analytical Chemistry, Denver, CO, August  2, 1999.

 

42.              “Characterization of the  Tryptophan Tryptophyl-Semiquinone Catalytic Intermediate of Methylamine Dehydrogenase,” 10th International Symposium on Vitamin B6 and Carbonyl Catalysis, Santa Fe, NM, October 31- November 5, 1999.

 

43.              "Mechanistic Implications of water coordination to two redox active enzymes" XIX International Meeting on Magnetic Resonance in Biological Systems, Florence, Italy, August 20-25, 2000.

 

44.              "Probing the membrane Binding Domain of COX-2 using Site-Directed Spin Labeling," XXth International Conference on Magnetic Resonance in Biological Systems, Toronto, CA, August 25-30, 2002.

 

45.              "Studies of Structure-Function Relationships in Cytochrome Oxidase using Pulse EPR Spectroscopy," College of Charleston, Charleston, SC, October 17, 2002.

 

46.              "Probing Product Release for Cytochrome Oxidase: a Freeze-Quench EPR Study," University of Iowa, Iowa City, IA, November 22, 2002.

 

47.              "Probing Product Release for Cytochrome Oxidase: a Freeze-Quench EPR Study," University of California, Davis, CA, March 13, 2003.

 

48.              “A Discrete Water Exit Pathway in Cytochrome c Oxidase,” 26th International EPR Symposium, Denver, CO, August 1-4, 2003.

 

49.              “A Discrete Water Exit Pathway in Cytochrome c Oxidase,” University of Michigan, Dearborn, MI November 11, 2003.

 

50.              “Characterization of the Cu(II) Binding Sites of FET3p Using EPR Methods,” International Society of Magnetic Resonance,  15th Meeting, Jacksonville, FL, October 24-30, 2004.

 

51.              “Characterization of the Cu(II) Binding Sites of FET3p Using EPR Methods,” Oakland University Chemistry Dept. Seminar, November 10, 2004.

 

52.              “Elucidation of Ligand Hyperfine Couplings for Multi-Copper Oxidases Using Two-Dimensional ESEEM Spectroscopy,” Southeastern Magnetic Resonance Conference, Atlanta, GA, November 12, 2005.

 

53.               “Elucidation of Ligand Hyperfine Couplings for Multi-Copper Oxidases Using Two-Dimensional ESEEM Spectroscopy,” University of Akron,  Department of Chemistry, November 29, 2005.

 

 

 

 

Presentations at Meetings

 

1.         McCracken, J. and Sauer, K., Orientation Dependence of Radical Pair Interactions in Spinach Chloroplasts.  Xth International Conference on Magnetic Resonance in Biological Systems, Stanford University, Stanford, California, August 1982.

 

2.         McCracken, J.; Magliozzo, R. S.; and Peisach, J., ESEEM Studies of Axial Ligation to Iron (III) Tetraphenylporphyrin Compounds.  8th International Symposium on Electron Paramagnetic Resonance, Denver, Colorado, August 1985.

 

3.         McCracken, J.; Dooley, D.; and Peisach, J., Pulsed electron paramagnetic resonance studies of copper binding sites in amine oxidases.  ASBC/ACS Meeting, Washington, D. C., June 1986.

 

4.         Peisach, J.; Alviggi, M.; and McCracken, J.,  The displacement of Cu(II)-bound water by cyanide in bovine superoxide dismutase.  Conference on Genetic Physico-Chemical Approaches for Analysis of Biological Catalysts, Florence, Italy, June 1986.

 

5.         McCracken, J.; Dooley, D.; and Peisach, J.,  Pulsed electron paramagnetic resonance studies of copper binding sites in amine oxidases.  28th Rocky Mountain Conference; 9th International Symposium on Electron Paramagnetic Resonance, Denver, Colorado, August 1986.

 

6.         Crowder, M.; McCracken, J.; Peisach, J.; and Kubota, S.,  CW and pulsed EPR studies of cyclic dipeptide Cu(II) complexes.  28th Rocky Mountain Conference; 9th International Symposium on Electron Paramagnetic Resonance, Denver, Colorado, August 1986.

 

7.         McCracken, J.; Pember, S.; Benkovic, S.; Villafranca, J.; and Peisach, J.,  Pulsed EPR studies of Cu(II)-imidazole interactions in phenylalanine hydroxylase.  Amer. Phys. Soc., New York City, March 1987.

 

8.         Banerjee, A.; McCracken, J. L.; Peisach, J.; and Cooperman, B. S.,  Divalent metal ion:  phosporyl ligand interaction in yeast inorganic pyrophosphatase.  Enzyme Mechanism Conf., Asilomar, Calif., January 1987.

 

9.         Banerjee, A.; McCracken, J. L.; Peisach, J.; and Cooperman, B. S.,  Divalent metal ion:  phosporyl ligand interaction in yeast inorganic pyrophosphatase.  ASBC Meeting, Philadelphia, Pennsylvania, June 1987.

 

10.       Peisach, J. and McCracken, J.,  Pulsed EPR in bioinorganic chemistry.  3rd International Conference on Bioinorganic Chemistry, Noordwijkerhout, The Netherlands, July 1987.

 

11.       Magliozzo, R.; McCracken, J.; and Peisach, J.,  Electron-nuclear coupling in nitrosyl heme proteins and in nitrosyl Fe(II) and oxy Co(II) tetraphenylporphyrin complexes.  3rd International Conference on Bioinorganic Chemistry, Noordwijkerhout, The Netherlands, July 1987.

 

12.       McCracken, J.; Serpersu, E. H.; Peisach, J.; and Mildvan, A. S.,  Pulsed EPR and proton relaxation studies of staphylococcal nuclease (SN) and its metal-coordination mutants:  Counting the water ligands on Mn(II).  FASEB Meeting, Las Vegas, Nevada, May 1988.

 

13.       Serpersu, E. H.; McCracken, J.; Peisach, J.; and Mildvan, A. S.,  Pulsed EPR and phosphorus relaxation studies of staphylococcal nuclease (SN) and its metal coordinating mutants:  Detection of phosphoryl ligands of the metal.  FASEB Meeting, Las Vegas, Nevada, May 1988.

 

14.       Tipton, P. A.; McCracken, J.; Cornelius, J.; and Peisach, J.,  Pulsed EPR studies of rabbit muscle pyruvate kinase.  FASEB Meeting, Las Vegas, Nevada, May 1988.

 

15.       De Francesco, R.; Edmondson, D. E.; McCracken, J.; and Peisach, J.,  Pulsed EPR studies of the FMN neutral semiquinone of azotobacter flavodoxin.  FASEB Meeting, Las Vegas, Nevada, May 1988.

 

16.       Cornelius, J. B.; McCracken, J.; Belford, R. L.; and Peisach, J.,  ESEEM studies of 14N and 15N pyridine weakly bound to copper benzac.  30th Rocky Mountain Conference, Denver, Colorado, July 1988.

 

17.       Jiang, F.; McCracken, J.; and Peisach, J.,  Electron-nuclear coupling in Cu(II) proteins and in Cu(II) diethylenetriamine complexes with substituted imidazoles.  XIII International Conference on Magnetic Resonance in Biological Systems, Madison, Wisconsin, August 1988.

 

18.       McCracken, J.; Cammack, R.; Chapman, A.; Weiner, J. H.; Cornelius, J. B.; and Peisach, J.,  Electron spin-echo studies of E. Coli fumarate reductase.  XIII International Conference on Magnetic Resonance in Biological Systems, Madison, Wisconsin, August 1988.

 

19.       Edmonson, D. E.; De Francesco, R.; McCracken, J.; and Peisach, J.,  Pulsed EPR studies of the FMN neutral semiquinone of azotobacter flavodoxin.  XIII International Conference on Magnetic Resonance in Biological Systems, Madison, Wisconsin, August 1988.

 

20.       Serpersu, E. H.; McCracken, J.; Peisach, J.; and Mildvan, A. S.,  NMR and pulsed EPR studies of staphylococcal nuclease (SN).  XIII International Conference on Magnetic Resonance in Biological Systems, Madison, Wisconsin, August 1988.

 

21.       Tipton, P. A.; McCracken, J.; Cornelius, J.; and Peisach, J.,  Electron spin echo envelope modulation studies of kinase active sites.  22nd International Conference on ESR of Inorganic Radicals and Metal Ions in Inorganic and Biological Systems, Sheffield, England, 1989.

 

22.       Magliozzo, R. S.; McCracken, J.; Corneli