Gavin Reid
Associate Professor Office: 234 Chemistry
Phone: 517-355-9715 198 /
Websites: Research Group - Area
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Bioanalytical Mass Spectrometry
(Research Description PDF - 974 kb)Research in the Reid laboratory is broadly directed toward the development and application of novel mass spectrometry based chemical methods and instrumentation for the selective identification, characterization and quantitative analysis of trace abundant bio-molecules involved in the regulation of cellular function, (e.g., amino acids, peptides, proteins, phospholipids).
Aspects of this research include:
(i) experimental, theoretical and statistical analysis of the gas-phase fragmentation pathways of biologically derived ions to determine the mechanisms and other factors influencing their dissociation reactions;
(ii) incorporation of the fragmentation "rules" arising from these studies into database search algorithms to help develop more effective search and scoring algorithms for use in automated high throughput identification strategies;
(ii) development of chemical derivatization reagents and associated methodologies to control and direct the dissociation of gas-phase bio-ions containing selected functional groups (for example, the side chain of a particular amino acids or a certain lipid head group) toward analytically useful fragmentation pathways; and
(iv) the development and application of `top down' proteomics strategies using sequence information derived from the gas-phase dissociation of intact protein ions to identify and quantify individual proteins or to characterize the nature of specific protein-protein, protein-ligand or protein-lipid interactions.
Selected Publications
The Origin and Control of Ex Vivo Oxidative Peptide Modifications Prior to Mass Spectrometry Analysis, Froelich, J.M. and Reid, G.E., Proteomics 2008, 8, 1334-1345.Mechanistic Insights into the Multistage Gas-Phase Fragmentation Behavior of Phosphoserine- and Phosphothreonine-containing Peptides, Palumbo, A.M., Tepe, J.J. and Reid, G.E., J. Proteome Res. 2008, 7, 771-779.
Metal-activated C-peptide Facilitates Glucose Clearance and the Release of a Nitric Oxide Stimulus via the GLUT1 Transporter, Meyer, J., Froelich, J.M., Reid, G.E., Karunarathne, W. and Spence, D., Diabetologia 2008, 51, 175-182.
Mechanisms for the Proton Mobility Dependant Gas-Phase Fragmentation Reactions of S-alkyl Cysteine Sulfoxide-containing Peptide Ions, Froelich J.M. and Reid, G.E., J. Am. Soc. Mass Spectrom. 2007, 18, 1690-1705.
Experimental and Theoretical Proton Affinities of Methionine, Methionine sulfoxide and their N- and C-terminal Derivatives, Lioe, H., O?Hair, R.A.J. Gronert, S., Austin, A. and Reid, G.E., Int. J. Mass Spectrom. 2007, 267, 220-232.
Emerging Methods in Proteomics: Multistage Tandem Mass Spectrometry for Top-down Protein Characterization, Scherperel, G. and Reid, G.E., The Analyst 2007, 132, 500-506.
